Fig. 6

Cryoablation suppressed lung cancer metastasis in mouse models. A, B Masson staining results displayed redeposition of collagen in the Con group, forming thick, dense, straight collagen fibers passing through tumor nests; collagen fibers in the Cry group appeared loose and curved structures surrounding tumor nests. n = 3 mice per group. Statistical significance was evaluated using one-way ANOVA. *P < 0.05, Con vs. Cry + SB431542. C Assessment of metastatic lesions in mouse lungs revealed minimal metastases in the cryoablation group, with a comparison showing 60% versus 0% metastatic incidence between the control and cryoablation groups, respectively. n = 5 mice per group